pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA
(Plasmid
#233031)
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PurposeTo express a DjCas13d-compatible gRNA
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDjCas13d-compatible gRNA expression cassette
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gRNA/shRNA sequenceUnknown
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SpeciesSynthetic
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains two U6-DRs; once you cut with SapI and ligate the gRNA into the plasmid there will only be one U6DR
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA was a gift from Jonathan Ploski (Addgene plasmid # 233031 ; http://n2t.net/addgene:233031 ; RRID:Addgene_233031)