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env8-AD-5'antiPNA-U1
(Plasmid #233011)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233011 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    U1 human
  • Backbone manufacturer
    Beckley et al., 2001 (doi: 10.1128/MCB.21.8.2815-2825.2001)
  • Total vector size (bp) 3634
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U1
  • Alt name
    NCBI Reference Sequence: NR_004430.2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    244
  • Mutation
    1 copy of Riboglow tag (truncated variant A with 5' antiPNA motif) near 5' end of U1
  • Entrez Gene
    RNU1-1 (a.k.a. HSD1, HU1-1, RNU1, RNU1A, RNU1A3, RNU1G4, Rnu1a1, U1, U1A1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BclI (not destroyed)
  • 5′ sequencing primer none tested
  • 3′ sequencing primer U1-H-Seq_RV (5'-GGAAAGCGCGAACGCAGTC-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    env8-AD-5'antiPNA-U1 was a gift from Amy Palmer (Addgene plasmid # 233011 ; http://n2t.net/addgene:233011 ; RRID:Addgene_233011)

  • For your References section:

    Unveiling the promise of peptide nucleic acids as functional linkers for an RNA imaging platform. Wierzba AJ, Richards EM, Lennon SR, Batey RT, Palmer AE. RSC Chem Biol. 2024 Dec 19. doi: 10.1039/d4cb00274a. 10.1039/d4cb00274a PubMed 39759445
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