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Addgene

pAG415Gal_Gcn4 R3W
(Plasmid #232975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAG415Gal-ccdB
  • Backbone manufacturer
    Susan Lindquist (Addgene #14145)
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gcn4 R++ W+
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    D103R, N126W, D133R, K143R
  • Promoter Gal1
  • Tags / Fusion Proteins
    • TEV cleavage site (N terminal on insert)
    • 3xHA (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG415Gal_Gcn4 R3W was a gift from Aseem Ansari & Tanja Mittag (Addgene plasmid # 232975 ; http://n2t.net/addgene:232975 ; RRID:Addgene_232975)

  • For your References section:

    Reconciling competing models on the roles of condensates and soluble complexes in transcription factor function. Bremer A, Lang WH, Kempen RP, Sweta K, Taylor AB, Borgia MB, Ansari AZ, Mittag T. bioRxiv 2024.11.21.624739 10.1101/2024.11.21.624739
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