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pLenti-EF1a-HIF2a dPA (P405A, P531A)
(Plasmid #232954)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentiEF1a
  • Backbone size w/o insert (bp) 9600
  • Total vector size (bp) 12213
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIF2a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2613
  • Mutation
    P405A, P531A
  • GenBank ID
    NM_001430.5
  • Entrez Gene
    EPAS1 (a.k.a. ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73)
  • Promoter EF1a

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-EF1a-HIF2a dPA (P405A, P531A) was a gift from William Kaelin (Addgene plasmid # 232954 ; http://n2t.net/addgene:232954 ; RRID:Addgene_232954)

  • For your References section:

    HIF regulates multiple translated endogenous retroviruses: Implications for cancer immunotherapy. Jiang Q, Braun DA, Clauser KR, Ramesh V, Shirole NH, Duke-Cohan JE, Nabilsi N, Kramer NJ, Forman C, Lippincott IE, Klaeger S, Phulphagar KM, Chea V, Kim N, Vanasse AP, Saad E, Parsons T, Carr-Reynolds M, Carulli I, Pinjusic K, Jiang Y, Li R, Syamala S, Rachimi S, Verzani EK, Stevens JD, Lane WJ, Camp SY, Meli K, Pappalardi MB, Herbert ZT, Qiu X, Cejas P, Long HW, Shukla SA, Van Allen EM, Choueiri TK, Churchman LS, Abelin JG, Gurer C, MacBeath G, Childs RW, Carr SA, Keskin DB, Wu CJ, Kaelin WG Jr. Cell. 2025 Feb 25:S0092-8674(25)00156-4. doi: 10.1016/j.cell.2025.01.046. 10.1016/j.cell.2025.01.046 PubMed 40023154
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