MSCV-NRAS(G12D)-Neo
(Plasmid
#232953)
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PurposeExpresses mutant form of NRAS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCV
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNRAS(G12D)-Neo
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SpeciesH. sapiens (human)
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MutationG12D
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Entrez GeneNRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-NRAS(G12D)-Neo was a gift from Jianjun Chen (Addgene plasmid # 232953 ; http://n2t.net/addgene:232953 ; RRID:Addgene_232953) -
For your References section:
YTHDF2 promotes ATP synthesis and immune evasion in B cell malignancies. Chen Z, Zeng C, Yang L, Che Y, Chen M, Sau L, Wang B, Zhou K, Chen Y, Qing Y, Shen C, Zhang T, Wunderlich M, Wu D, Li W, Wang K, Leung K, Sun M, Tang T, He X, Zhang L, Swaminathan S, Mulloy JC, Muschen M, Huang H, Weng H, Xiao G, Deng X, Chen J. Cell. 2024 Dec 12:S0092-8674(24)01324-2. doi: 10.1016/j.cell.2024.11.007. 10.1016/j.cell.2024.11.007 PubMed 39694037