MSCV-BCR-ABL1
(Plasmid
#232952)
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PurposeExpresses BCR-ABL1 fusion protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCV
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBCR-ABL1
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SpeciesH. sapiens (human)
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Entrez GeneBCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: This plasmid contains N796S and N928K mutations in BCR and a T1065S mutation in ABL1 compared to their NCBI reference sequences. These mutations are not known to impact plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-BCR-ABL1 was a gift from Jianjun Chen (Addgene plasmid # 232952 ; http://n2t.net/addgene:232952 ; RRID:Addgene_232952) -
For your References section:
YTHDF2 promotes ATP synthesis and immune evasion in B cell malignancies. Chen Z, Zeng C, Yang L, Che Y, Chen M, Sau L, Wang B, Zhou K, Chen Y, Qing Y, Shen C, Zhang T, Wunderlich M, Wu D, Li W, Wang K, Leung K, Sun M, Tang T, He X, Zhang L, Swaminathan S, Mulloy JC, Muschen M, Huang H, Weng H, Xiao G, Deng X, Chen J. Cell. 2024 Dec 12:S0092-8674(24)01324-2. doi: 10.1016/j.cell.2024.11.007. 10.1016/j.cell.2024.11.007 PubMed 39694037