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Addgene

pCDH-3_Flag-TET1-S-MUT
(Plasmid #232943)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232943 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDH
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TET1-S-MUT
  • Species
    H. sapiens (human)
  • Mutation
    H1652Y and D1654A
  • Entrez Gene
    TET1 (a.k.a. CXXC6, LCX, bA119F7.1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains an I452M mutation in Tet1. This mutation is not known to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-3_Flag-TET1-S-MUT was a gift from Jianjun Chen (Addgene plasmid # 232943 ; http://n2t.net/addgene:232943 ; RRID:Addgene_232943)
  • For your References section:

    Phosphorylation stabilized TET1 acts as an oncoprotein and therapeutic target in B cell acute lymphoblastic leukemia. Chen Z, Zhou K, Xue J, Small A, Xiao G, Nguyen LXT, Zhang Z, Prince E, Weng H, Huang H, Zhao Z, Qing Y, Shen C, Li W, Han L, Tan B, Su R, Qin H, Li Y, Wu D, Gu Z, Ngo VN, He X, Chao J, Leung K, Wang K, Dong L, Qin X, Cai Z, Sheng Y, Chen Y, Wu X, Zhang B, Shi Y, Marcucci G, Qian Z, Xu M, Muschen M, Chen J, Deng X. Sci Transl Med. 2023 Mar 29;15(689):eabq8513. doi: 10.1126/scitranslmed.abq8513. Epub 2023 Mar 29. 10.1126/scitranslmed.abq8513 PubMed 36989375