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pCoofy1-psHaloTag1a
(Plasmid #232940)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232940 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCoofy1
  • Backbone manufacturer
    EMBL
  • Backbone size w/o insert (bp) 5334
  • Total vector size (bp) 6648
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    photoswitchable HaloTag 1a variant
  • Alt name
    psHaloTag1a
  • Insert Size (bp)
    1371
  • Mutation
    E143W, A258W with linkers L1 (FAG) and L2 (P)
  • Promoter T7
  • Tag / Fusion Protein
    • His6x-HRV3C (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG (T7 promoter, forward primer)
  • 3′ sequencing primer CTAGTTATTGCTCAGCGGT (T7 terminator, reverse primer)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.18.629107 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCoofy1-psHaloTag1a was a gift from Claire Deo (Addgene plasmid # 232940 ; http://n2t.net/addgene:232940 ; RRID:Addgene_232940)

  • For your References section:

    A photoswitchable HaloTag for spatiotemporal control of fluorescence in living cells. Walterspiel F, Ugarte-Uribe B, Weidenhausen J, Dimitriadi A, Khan AUM, Müller CW, Deo C. bioRxiv 2024.12.18.629107 10.1101/2024.12.18.629107
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