KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA
(Plasmid
#232935)
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PurposeDonor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScript II SK (+)
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Backbone manufacturerAgilent #212207
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Vector typeMouse Targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameT2A-eGFP-SV40pA with Grb10 homology arms
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gtaaaacgacggccagt (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA was a gift from Danny Reinberg (Addgene plasmid # 232935 ; http://n2t.net/addgene:232935 ; RRID:Addgene_232935) -
For your References section:
CTCF-RNA interactions orchestrate cell-specific chromatin loop organization. Lucero K, Han S, Huang P, Qiu X, Mazzoni EO, Reinberg D. bioRxiv, March 19, 2025 10.1101/2025.03.19.643339