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PX459-sgRNA048_Grb10
(Plasmid #232934)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232934 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX459
  • Backbone manufacturer
    Feng Zhang (Addgene #62988)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9-T2A-PuroR
  • gRNA/shRNA sequence
    gcctgtgcacattctcaggt
  • Species
    S. pyogenes
  • Entrez Gene
    Grb10 (a.k.a. 5730571D09Rik, Meg1, mKIAA0207)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PX459-sgRNA048_Grb10 was a gift from Danny Reinberg (Addgene plasmid # 232934 ; http://n2t.net/addgene:232934 ; RRID:Addgene_232934)

  • For your References section:

    CTCF-RNA interactions orchestrate cell-specific chromatin loop organization. Lucero K, Han S, Huang P, Qiu X, Mazzoni EO, Reinberg D. bioRxiv, March 19, 2025 10.1101/2025.03.19.643339
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