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Addgene

GAG-PEmax V7
(Plasmid #232921)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232921 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3-like
  • Total vector size (bp) 12313
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAG FMLV fused to PEmax
  • Alt name
    Editor V7
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    5877
  • Tag / Fusion Protein
    • Nuclear export signals (x3) (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Philippe Mangeot, Théophile Ohlmann and Emiliano Ricci (backbone derived from BIC-CAS9; Addgene #119942), and David Liu (PEmax amplified from Addgene #174820).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene observed 1 base pair mismatch in the ORF leading to H102Y substitution. This discrepancy was found to not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GAG-PEmax V7 was a gift from Philippe Mangeot (Addgene plasmid # 232921 ; http://n2t.net/addgene:232921 ; RRID:Addgene_232921)

  • For your References section:

    Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles. Halegua T, Risson V, Carras J, Rouyer M, Coudert L, Jacquier A, Schaeffer L, Ohlmann T, Mangeot PE. Nat Commun. 2025 Jan 4;16(1):397. doi: 10.1038/s41467-024-55604-0. 10.1038/s41467-024-55604-0 PubMed 39755699
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