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Addgene

pp-CBBC-Fluc
(Plasmid #232920)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232920 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHCMV-Firefly-Luciferase
  • Modifications to backbone
    insertion of Csy4 hairpins within intron
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter CMV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pp-CBBC-Fluc was a gift from Philippe Mangeot (Addgene plasmid # 232920 ; http://n2t.net/addgene:232920 ; RRID:Addgene_232920)
  • For your References section:

    Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles. Halegua T, Risson V, Carras J, Rouyer M, Coudert L, Jacquier A, Schaeffer L, Ohlmann T, Mangeot PE. Nat Commun. 2025 Jan 4;16(1):397. doi: 10.1038/s41467-024-55604-0. 10.1038/s41467-024-55604-0 PubMed 39755699