pp-CBBC-Fluc
(Plasmid
#232920)
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Purpose(Empty Backbone) Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHCMV-Firefly-Luciferase
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Modifications to backboneinsertion of Csy4 hairpins within intron
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Vector typeMammalian Expression, CRISPR
- Promoter CMV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pp-CBBC-Fluc was a gift from Philippe Mangeot (Addgene plasmid # 232920 ; http://n2t.net/addgene:232920 ; RRID:Addgene_232920) -
For your References section:
Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles. Halegua T, Risson V, Carras J, Rouyer M, Coudert L, Jacquier A, Schaeffer L, Ohlmann T, Mangeot PE. Nat Commun. 2025 Jan 4;16(1):397. doi: 10.1038/s41467-024-55604-0. 10.1038/s41467-024-55604-0 PubMed 39755699
Map uploaded by the depositor.
