pRS313-rad53-5004
(Plasmid
#232902)
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PurposePlasmid carrying the rad53-5004 allele with its native promoter and terminator.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232902 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS313
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Backbone manufacturerSikorski and Hieter, 1989, Genetics
- Backbone size w/o insert (bp) 4967
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAD53
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Alt nameYPL153C
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3318
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MutationT600C, A680G, A1094T, C1615T, C2228A
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Entrez GeneRAD53 (a.k.a. YPL153C, LSD1, MEC2, SPK1)
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS313-rad53-5004 was a gift from Jolanda Van Leeuwen (Addgene plasmid # 232902 ; http://n2t.net/addgene:232902 ; RRID:Addgene_232902)