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Addgene

pRS313-rad53-5004
(Plasmid #232902)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232902 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS313
  • Backbone manufacturer
    Sikorski and Hieter, 1989, Genetics
  • Backbone size w/o insert (bp) 4967
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAD53
  • Alt name
    YPL153C
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3318
  • Mutation
    T600C, A680G, A1094T, C1615T, C2228A
  • Entrez Gene
    RAD53 (a.k.a. YPL153C, LSD1, MEC2, SPK1)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS313-rad53-5004 was a gift from Jolanda Van Leeuwen (Addgene plasmid # 232902 ; http://n2t.net/addgene:232902 ; RRID:Addgene_232902)