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Addgene

pML107-YFR054C
(Plasmid #232900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pML107
  • Backbone manufacturer
    Laughery et al, 2015, Yeast
  • Backbone size w/o insert (bp) 12367
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFR054C gRNA
  • Alt name
    YFR054C
  • gRNA/shRNA sequence
    GCTCAAAGAAACAATTAGAG
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    33
  • Entrez Gene
    YFR054C (a.k.a. YFR054C)
  • Promoter snR52

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SwaI (destroyed during cloning)
  • 3′ cloning site BclI (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML107-YFR054C was a gift from Jolanda Van Leeuwen (Addgene plasmid # 232900 ; http://n2t.net/addgene:232900 ; RRID:Addgene_232900)
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