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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 23289 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV4
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Backbone manufacturerDavid Russells lab
- Backbone size w/o insert (bp) 4900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha, HB101, DM1,
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep52
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Alt nameNFKB2
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SpeciesH. sapiens (human)
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Insert Size (bp)1400
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Entrez GeneNFKB2 (a.k.a. CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p49/p100, p52)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV4-p52 was a gift from Warner Greene (Addgene plasmid # 23289 ; http://n2t.net/addgene:23289 ; RRID:Addgene_23289) -
For your References section:
Human T-cell leukemia virus type I Tax associates with and is negatively regulated by the NF-kappa B2 p100 gene product: implications for viral latency. Beraud C, Sun SC, Ganchi P, Ballard DW, Greene WC. Mol Cell Biol. 1994 Feb . 14(2):1374-82. PubMed 8289813