pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}
(Plasmid
#232880)
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PurposeExpresses the ARL13B coupled to GFP and RGECO1 calcium sensor.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV[Exp]-Puro-CMV
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Backbone manufacturerVector Builder
- Total vector size (bp) 11238
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameADP-ribosylation factor-like protein 13B
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Alt nameARL13B
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SpeciesH. sapiens (human)
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Insert Size (bp)3332
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Entrez GeneARL13B (a.k.a. ARL2L1, JBTS8)
- Promoter CMV
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Tags
/ Fusion Proteins
- GFP (C terminal on insert)
- RGECO1 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CGC AAA TGG GCG GTA GGC GTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1} was a gift from Paul DeCaen (Addgene plasmid # 232880 ; http://n2t.net/addgene:232880 ; RRID:Addgene_232880) -
For your References section:
Methods to Assess Neuronal Primary Cilia Electrochemical Signaling. DeCaen PG, Kimura LF. J Cell Physiol. 2025 Apr;240(4):e70034. doi: 10.1002/jcp.70034. 10.1002/jcp.70034 PubMed 40227694