pCMV4-Tax(M20)
(Plasmid
#23285)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV4
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Backbone manufacturerDavid Russells lab
- Backbone size w/o insert (bp) 4900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha DM1 HB101
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTax(M20)
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Speciestype I human T-cell leukemia virus (HTLV-I)
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MutationT130A L131S
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV4-Tax(M20) was a gift from Warner Greene (Addgene plasmid # 23285 ; http://n2t.net/addgene:23285 ; RRID:Addgene_23285) -
For your References section:
Identification of HTLV-I tax trans-activator mutants exhibiting novel transcriptional phenotypes. Smith MR, Greene WC. Genes Dev. 1990 Nov . 4(11):1875-85. 10.1101/gad.4.11.1875 PubMed 2276622