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pEW108
(Plasmid #232823)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232823 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBIG2ab
  • Backbone size w/o insert (bp) 5669
  • Total vector size (bp) 18708
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    12793
  • Mutation
    Set1(762-1080)
  • Entrez Gene
    BRE2 (a.k.a. YLR015W, CPS60)
  • Entrez Gene
    SWD1 (a.k.a. YAR003W, CPS50, FUN16, SAF49)
  • Entrez Gene
    SET1 (a.k.a. YHR119W, KMT2, YTX1)
  • Entrez Gene
    SPP1 (a.k.a. YPL138C, CPS40, SAF41)
  • Entrez Gene
    SWD3 (a.k.a. YBR175W, CPS30, SAF35)
  • Entrez Gene
    SWD2 (a.k.a. YKL018W, CPS35, SAF37)
  • Entrez Gene
    SDC1 (a.k.a. YDR469W, CPS25, SAF19)
  • Entrez Gene
    SHG1 (a.k.a. YBR258C, CPS15)
  • Promoter polyhedrin promoter
  • Tag / Fusion Protein
    • His6-3xFLAG, Twinstrep (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEW108 was a gift from Cynthia Wolberger (Addgene plasmid # 232823 ; http://n2t.net/addgene:232823 ; RRID:Addgene_232823)

  • For your References section:

    Structural basis for COMPASS recognition of an H2B-ubiquitinated nucleosome. Worden EJ, Zhang X, Wolberger C. Elife. 2020 Jan 10;9. pii: 53199. doi: 10.7554/eLife.53199. 10.7554/eLife.53199 PubMed 31922488
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