pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP
(Plasmid
#232800)
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PurposeHelper plasmid to trigger auxin-inducible-degradation of targeted proteins.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDH
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Vector typeLentiviral
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Selectable markersRFP and EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOsTIR1 F74G EGFP-AID
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SpeciesOryza sativa
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer plasmidsaurus full plasmid sequencing (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP was a gift from Chunliang Li (Addgene plasmid # 232800 ; http://n2t.net/addgene:232800 ; RRID:Addgene_232800) -
For your References section:
Auxin-inducible degron 2 system deciphers functions of CTCF domains in transcriptional regulation. Hyle J, Djekidel MN, Williams J, Wright S, Shao Y, Xu B, Li C. Genome Biol. 2023 Jan 26;24(1):14. doi: 10.1186/s13059-022-02843-3. 10.1186/s13059-022-02843-3 PubMed 36698211
Map uploaded by the depositor.
