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Addgene

PHB2-GFP
(Plasmid #232788)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232788 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHB2
  • Alt name
    REA
  • Alt name
    BAP
  • Species
    H. sapiens (human)
  • Entrez Gene
    PHB2 (a.k.a. BAP, BCAP37, Bap37, PNAS-141, REA, hBAP, p22)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BshtI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PHB2-GFP was a gift from Giulia Bertolin (Addgene plasmid # 232788 ; http://n2t.net/addgene:232788 ; RRID:Addgene_232788)

  • For your References section:

    Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin 2. Bertolin G, Alves-Guerra MC, Cheron A, Burel A, Prigent C, Le Borgne R, Tramier M. Life Sci Alliance. 2021 Apr 5;4(6):e202000806. doi: 10.26508/lsa.202000806. Print 2021 Jun. 10.26508/lsa.202000806 PubMed 33820826
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