PHB2 S39A-mCherry
(Plasmid
#232786)
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PurposeEncodes an mCherry-tagged Prohibitin2/PHB2 non-phosphorylatable on Ser39
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232786 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePHB2
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Alt nameREA
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Alt nameBAP
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SpeciesH. sapiens (human)
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MutationChanged Ser 39 to Ala
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Entrez GenePHB2 (a.k.a. BAP, BCAP37, Bap37, PNAS-141, REA, hBAP, p22)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (C terminal on backbone)
Cloning Information
- Cloning method Other
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene observed E229G substitution in the PHB2 insert. This discrepancy should not affect the plasmid functionality.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PHB2 S39A-mCherry was a gift from Giulia Bertolin (Addgene plasmid # 232786 ; http://n2t.net/addgene:232786 ; RRID:Addgene_232786) -
For your References section:
Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin 2. Bertolin G, Alves-Guerra MC, Cheron A, Burel A, Prigent C, Le Borgne R, Tramier M. Life Sci Alliance. 2021 Apr 5;4(6):e202000806. doi: 10.26508/lsa.202000806. Print 2021 Jun. 10.26508/lsa.202000806 PubMed 33820826