PHB1-mCherry
(Plasmid
#232784)
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PurposeEncodes an mCherry-tagged Prohibitin1/PHB1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232784 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePHB1
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Alt namePHB
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SpeciesH. sapiens (human)
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Insert Size (bp)816
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Entrez GenePHB1 (a.k.a. BAP32, HEL-215, HEL-S-54e, PHB)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PHB1-mCherry was a gift from Giulia Bertolin (Addgene plasmid # 232784 ; http://n2t.net/addgene:232784 ; RRID:Addgene_232784) -
For your References section:
Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin 2. Bertolin G, Alves-Guerra MC, Cheron A, Burel A, Prigent C, Le Borgne R, Tramier M. Life Sci Alliance. 2021 Apr 5;4(6):e202000806. doi: 10.26508/lsa.202000806. Print 2021 Jun. 10.26508/lsa.202000806 PubMed 33820826