pAM-FLEX-hM4D(Gi)-mCherry
(Plasmid #232782)

• Purpose: AAV2 vector containing Gi-coupled hM4D DREADD fused with mCherry inside a double floxed (FLEX) cassette under the control of CBA promoter.
• Depositing Lab: Peer Wulff
• Publication: Baccini et al bioRxiv 2024.08.13.606964

Backbone

• Vector backbone: pAM
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hM4D(Gi)-mCherry
• Species: Synthetic

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: gcgagatcaagcagagg
• 3′ sequencing primer: gcgtaaaaggagcaacata

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Bryan Roth (Addgene plasmid #44362), Andrew Murray (Nat. Neurosci. 2011 Mar;14(3):297-9. doi: 10.1038/nn.2751), Matthias Klugmann (Mol. Cell. Neurosci. 2005, 28:343-360 https://doi.org/10.1016/j.mcn.2004.10.002).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The AAV2 backbone containing the 1.1-kb CMV enhancer/chicken h-actin (CBA) promoter, the woodchuck post-transcriptional regulatory element (WPRE), and the bovine growth hormone polyA was derived from a plasmid made by Matthias Klugmann (Mol. Cell. Neurosci. 2005, 28:343-360 https://doi.org/10.1016/j.mcn.2004.10.002)

The Flex cassette was introduced by Andrew Murray (Nat. Neurosci. 2011 Mar;14(3):297-9. doi: 10.1038/nn.2751).

The Gi-coupled hM4D DREADD fused with mCherry was derived from Addgene plasmid #44362 using the NheI and AscI restriction enzymes and cloned into the EcoRI (filled-in) and SpeI restriction sites of the empty pAM-Flex vector, thus destroying the SpeI restriction site while preserving the EcoRI site.

Please visit https://doi.org/10.1101/2024.08.13.606964 for bioRxiv preprint.