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Addgene

dCas9-NONO1_IDR-EGFP
(Plasmid #232764)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232764 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiCRISPRv2
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-NONO1_IDR-EGFP
  • Species
    H. sapiens (human)
  • Entrez Gene
    NONO (a.k.a. MRXS34, NMT55, NRB54, P54, P54NRB, PPP1R114)
  • Promoter EF1a

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dCas9-NONO1_IDR-EGFP was a gift from Isaac Hilton (Addgene plasmid # 232764 ; http://n2t.net/addgene:232764 ; RRID:Addgene_232764)

  • For your References section:

    Biomolecular condensation of human IDRs initiates endogenous transcription via intrachromosomal looping or high-density promoter localization. Li J, Liu S, Kim S, Goell J, Drum ZA, Flores JP, Ma AJ, Mahata B, Escobar M, Raterink A, Ahn JH, Teran ER, Guerra-Resendez RS, Zhou Y, Yu B, Diehl MR, Wang GG, Gustavsson AK, Phanstiel DH, Hilton IB. Nucleic Acids Res. 2025 Feb 8;53(4):gkaf056. doi: 10.1093/nar/gkaf056. 10.1093/nar/gkaf056 PubMed 39933697
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