pcDNA3.1(+) NAPstar3b
(Plasmid
#232736)
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PurposeExpresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232736 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
- Backbone size w/o insert (bp) 8182
- Total vector size (bp) 5422
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNAPstar3b
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SpeciesH. sapiens (human)
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Insert Size (bp)2760
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+) NAPstar3b was a gift from Bruce Morgan (Addgene plasmid # 232736 ; http://n2t.net/addgene:232736 ; RRID:Addgene_232736) -
For your References section:
A family of NADPH/NADP(+) biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes. Scherschel M, Niemeier JO, Jacobs LJHC, Hoffmann MDA, Diederich A, Bell C, Hohne P, Raetz S, Kroll JB, Steinbeck J, Lichtenauer S, Multhoff J, Zimmermann J, Sadhanasatish T, Rothemann RA, Grashoff C, Messens J, Ampofo E, Laschke MW, Riemer J, Roma LP, Schwarzlander M, Morgan B. Nat Commun. 2024 Dec 19;15(1):10704. doi: 10.1038/s41467-024-55302-x. 10.1038/s41467-024-55302-x PubMed 39702652