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Addgene

pX459_CDC45_sgRNA
(Plasmid #232732)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232732 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX459
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDC45
  • gRNA/shRNA sequence
    tgtc ctagg gtgag ttac ag
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Mutation
    NO
  • Entrez Gene
    CDC45 (a.k.a. CDC45L, CDC45L2, MGORS7, PORC-PI-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site hU6-F (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX459_CDC45_sgRNA was a gift from Junjie Chen (Addgene plasmid # 232732 ; http://n2t.net/addgene:232732 ; RRID:Addgene_232732)
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