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Addgene

pX459_MCM2_sgRNA
(Plasmid #232730)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232730 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX330
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCM2
  • gRNA/shRNA sequence
    gagg cccta tgcca tccata
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Mutation
    NO
  • Entrez Gene
    MCM2 (a.k.a. BM28, CCNL1, CDCL1, D3S3194, DFNA70, MITOTIN, cdc19)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site hU6-F (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX459_MCM2_sgRNA was a gift from Junjie Chen (Addgene plasmid # 232730 ; http://n2t.net/addgene:232730 ; RRID:Addgene_232730)
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