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PE-pegRNA(BG)-nsgRNA(BG)
(Plasmid #232727)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232727 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
  • gRNA/shRNA sequence
    pegRNA(BG): CACCGCTCGTGACCACCCTGACCCA; nsgRNA(BG): CACCGGACGTAGCCTTCGGGCATGG;
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The U6 promoters are from pHSG1C3 (Plasmid #164423).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PE-pegRNA(BG)-nsgRNA(BG) was a gift from David Brafman (Addgene plasmid # 232727 ; http://n2t.net/addgene:232727 ; RRID:Addgene_232727)

  • For your References section:

    PINE-TREE enables highly efficient genetic modification of human cell lines. Frisch C, Kostes WW, Galyon B, Whitman B, Tekel SJ, Standage-Beier K, Srinivasan G, Wang X, Brafman DA. Mol Ther Nucleic Acids. 2023 Jul 17;33:483-492. doi: 10.1016/j.omtn.2023.07.007. eCollection 2023 Sep 12. 10.1016/j.omtn.2023.07.007 PubMed 37588683
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