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Addgene

PLK3 1-334 T219D-pEGFP
(Plasmid #23268)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23268 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PLK3
  • Alt name
    CNK
  • Alt name
    FNK
  • Alt name
    PRK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1938
  • Mutation
    aa 1-334 T219D
  • Entrez Gene
    PLK3 (a.k.a. CNK, FNK, PLK-3, PRK)
  • Tag / Fusion Protein
    • pEGFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLK3 1-334 T219D-pEGFP was a gift from Wei Dai (Addgene plasmid # 23268 ; http://n2t.net/addgene:23268 ; RRID:Addgene_23268)
  • For your References section:

    Polo-like kinase 3 functions as a tumor suppressor and is a negative regulator of hypoxia-inducible factor-1 alpha under hypoxic conditions. Yang Y, Bai J, Shen R, Brown SA, Komissarova E, Huang Y, Jiang N, Alberts GF, Costa M, Lu L, Winkles JA, Dai W. Cancer Res. 2008 Jun 1. 68(11):4077-85. 10.1158/0008-5472.CAN-07-6182 PubMed 18519666