Skip to main content
Addgene

pXJ-His-VTCN1
(Plasmid #232664)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232664 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    modified pXJ40
  • Backbone size w/o insert (bp) 4616
  • Vector type
    Mammalian Expression
  • Selectable markers
    No mammalian selection marker

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VTCN1
  • Alt name
    B7-H4
  • Alt name
    B7X
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    882
  • Entrez Gene
    VTCN1 (a.k.a. B7-H4, B7H4, B7S1, B7X, B7h.5, PRO1291, VCTN1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • His (retained after signal peptide cleavage) (N terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXJ-His-VTCN1 was a gift from Chen-Yang Shen (Addgene plasmid # 232664 ; http://n2t.net/addgene:232664 ; RRID:Addgene_232664)

  • For your References section:

    Genetic insights into carbohydrate sulfotransferase 8 and its impact on the immunotherapy efficacy of cancer. Chou WC, Chen WT, Kuo CT, Chang YM, Lu YS, Li CW, Hung MC, Shen CY. Cell Rep. 2024 Jan 23;43(1):113641. doi: 10.1016/j.celrep.2023.113641. Epub 2023 Dec 30. 10.1016/j.celrep.2023.113641 PubMed 38165805
Related items: