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pXJ-Nluc-_(PLRSKK)-CA1-19
(Plasmid #232657)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232657 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pXJ40
  • Backbone size w/o insert (bp) 4612
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    No mammalian selection marker

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CGA
  • Alt name
    CG-ALPHA
  • Alt name
    LHA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    967
  • Entrez Gene
    CGA (a.k.a. CG-ALPHA, FSHA, GPA1, GPHA1, GPHa, HCG, LHA, TSHA)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    from synthetic gene in pBluescript II SK(+) vector

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXJ-Nluc-_(PLRSKK)-CA1-19 was a gift from Chen-Yang Shen (Addgene plasmid # 232657 ; http://n2t.net/addgene:232657 ; RRID:Addgene_232657)

  • For your References section:

    Genetic insights into carbohydrate sulfotransferase 8 and its impact on the immunotherapy efficacy of cancer. Chou WC, Chen WT, Kuo CT, Chang YM, Lu YS, Li CW, Hung MC, Shen CY. Cell Rep. 2024 Jan 23;43(1):113641. doi: 10.1016/j.celrep.2023.113641. Epub 2023 Dec 30. 10.1016/j.celrep.2023.113641 PubMed 38165805
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