pXJ-Myc-KLF16
(Plasmid
#232655)
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Purposetransient expresses KLF16 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232655 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemodified pXJ40
- Backbone size w/o insert (bp) 4669
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Vector typeMammalian Expression
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Selectable markersNo mammalian selection marker
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKLF16
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SpeciesH. sapiens (human)
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Insert Size (bp)763
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Entrez GeneKLF16 (a.k.a. BTEB4, DRRF, NSLP2)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer Bglob-intron-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXJ-Myc-KLF16 was a gift from Chen-Yang Shen (Addgene plasmid # 232655 ; http://n2t.net/addgene:232655 ; RRID:Addgene_232655) -
For your References section:
Genetic insights into carbohydrate sulfotransferase 8 and its impact on the immunotherapy efficacy of cancer. Chou WC, Chen WT, Kuo CT, Chang YM, Lu YS, Li CW, Hung MC, Shen CY. Cell Rep. 2024 Jan 23;43(1):113641. doi: 10.1016/j.celrep.2023.113641. Epub 2023 Dec 30. 10.1016/j.celrep.2023.113641 PubMed 38165805