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Addgene

pXJ-Myc-FOXA1
(Plasmid #232645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pXJ40
  • Backbone size w/o insert (bp) 4669
  • Vector type
    Mammalian Expression
  • Selectable markers
    No mammalian selection marker

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FOXA1
  • Alt name
    HNF3A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1422
  • Entrez Gene
    FOXA1 (a.k.a. HNF3A, TCF3A)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXJ-Myc-FOXA1 was a gift from Chen-Yang Shen (Addgene plasmid # 232645 ; http://n2t.net/addgene:232645 ; RRID:Addgene_232645)

  • For your References section:

    A common variant in 11q23.3 associated with hyperlipidemia is mediated by the binding and regulation of GATA4. Chou WC, Chen WT, Shen CY. NPJ Genom Med. 2022 Jan 19;7(1):4. doi: 10.1038/s41525-021-00279-5. 10.1038/s41525-021-00279-5 PubMed 35046404
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