pCS2-Opto-Acvr1ba-P2A-Acvr2ba
(Plasmid
#232640)
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PurposeOptogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232640 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
- Total vector size (bp) 7437
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
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SpeciesD. rerio (zebrafish); Vaucheria frigida
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Insert Size (bp)1893
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Based on the constructs first developed by Sako et al. 2016 (Cell Reports, https://doi.org/10.1016/j.celrep.2016.06.036)
Please visit https://doi.org/10.1101/2025.04.17.649426 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-Opto-Acvr1ba-P2A-Acvr2ba was a gift from Katherine Rogers (Addgene plasmid # 232640 ; http://n2t.net/addgene:232640 ; RRID:Addgene_232640)