pCS2-Opto-zFGFR1a
(Plasmid
#232639)
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PurposeZebrafish FGFR1a receptor kinase domain fused to VfLOV
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232639 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
- Total vector size (bp) 5979
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namezFGFR1a kinase domain + VfLOV domain
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SpeciesD. rerio (zebrafish); Vaucheria frigida
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Insert Size (bp)1893
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Based on the chassis first developed by Sako et al. 2016 (Cell Reports, https://doi.org/10.1016/j.celrep.2016.06.036) and later used in Rogers et al. 2020 (eLife, https://doi.org/10.7554/elife.58641)
Please visit https://doi.org/10.1101/2025.04.17.649426 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-Opto-zFGFR1a was a gift from Katherine Rogers (Addgene plasmid # 232639 ; http://n2t.net/addgene:232639 ; RRID:Addgene_232639)