HaloTag-LacI #1691
(Plasmid
#232636)
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Purposelac repressor (LacI) which binds lacO sites; labeled with HaloTag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 232636 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHTN-HaloTag
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 6143
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHaloTag-LacI
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SpeciesSynthetic
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Mutationnone
- Promoter CMV
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Tag
/ Fusion Protein
- NLS (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.12.09.627308 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HaloTag-LacI #1691 was a gift from Karsten Rippe (Addgene plasmid # 232636 ; http://n2t.net/addgene:232636 ; RRID:Addgene_232636)