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Addgene

delta-LIM Isl1
(Plasmid #23261)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23261 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4097
  • Vector type
    Mammalian Expression ; avian/xenopus/zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Isl1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    720
  • Mutation
    Delta LIM
  • Entrez Gene
    Isl1 (a.k.a. Isl-1, isl-1=homeobox)
  • Tag / Fusion Protein
    • Lamda-dimer domain (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F, SP6
  • 3′ sequencing primer SV40-Pa-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See map for fusion details.

Plasmid #321 of the Pfaff Lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    delta-LIM Isl1 was a gift from Samuel Pfaff (Addgene plasmid # 23261 ; http://n2t.net/addgene:23261 ; RRID:Addgene_23261)

  • For your References section:

    LIM factor Lhx3 contributes to the specification of motor neuron and interneuron identity through cell-type-specific protein-protein interactions. Thaler JP, Lee SK, Jurata LW, Gill GN, Pfaff SL. Cell. 2002 Jul 26. 110(2):237-49. 10.1016/S0092-8674(02)00823-1 PubMed 12150931
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