pEHAC1-CHIP-BirA-HA
(Plasmid
#232587)
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PurposeExpress BirA tagged to the C-terminus of CHIP.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEHAC1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCHIP - BirA
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SpeciesH. sapiens (human)
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Entrez GeneCHIP
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEHAC1-CHIP-BirA-HA was a gift from Sagar Bhogaraju (Addgene plasmid # 232587 ; http://n2t.net/addgene:232587 ; RRID:Addgene_232587) -
For your References section:
A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates. Mukhopadhyay U, Levantovsky S, Carusone TM, Gharbi S, Stein F, Behrends C, Bhogaraju S. Sci Adv. 2024 Aug 9;10(32):eadp3000. doi: 10.1126/sciadv.adp3000. Epub 2024 Aug 9. 10.1126/sciadv.adp3000 PubMed 39121224