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Addgene

pIRESpuro-Flag-Pol b(K72A)
(Plasmid #23258)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23258 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESpuroGWB
  • Backbone manufacturer
    Clontech, modifed by Sobol Lab
  • Backbone size w/o insert (bp) 7100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNA polymerase beta (K72A)
  • Alt name
    POLB (K72A)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1263
  • Mutation
    K72A
  • Entrez Gene
    POLB
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRESpuro-Flag-Pol b(K72A) was a gift from Robert Sobol (Addgene plasmid # 23258 ; http://n2t.net/addgene:23258 ; RRID:Addgene_23258)

  • For your References section:

    Bioenergetic Metabolites Regulate Base Excision Repair-Dependent Cell Death in Response to DNA Damage. Tang JB, Goellner EM, Wang XH, Trivedi RN, St Croix CM, Jelezcova E, Svilar D, Brown AR, Sobol RW. Mol Cancer Res. 2010 Jan 12. ():. 10.1158/1541-7786.MCR-09-0411 PubMed 20068071
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