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Addgene

pcDNA3.1-300(HAT-)
(Plasmid #23254)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23254 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 (-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p300
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7245
  • Mutation
    H1415A E1423A Y1424A L1428S Y1430A H1434A
  • Entrez Gene
    EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-300(HAT-) was a gift from Warner Greene (Addgene plasmid # 23254 ; http://n2t.net/addgene:23254 ; RRID:Addgene_23254)

  • For your References section:

    Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-kappaB. Chen LF, Mu Y, Greene WC. EMBO J. 2002 Dec 2. 21(23):6539-48. 10.1093/emboj/cdf660 PubMed 12456660
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