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Addgene

T7-RelA(K218R)
(Plasmid #23247)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEv3s (modified pET3A)
  • Backbone manufacturer
    Matthias P et al. Nucleic Acids Research (1989). 17(15): 6418
  • Backbone size w/o insert (bp) 5120
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a @ 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFkB RelA(K218R)
  • Alt name
    T7-RelA(K218R)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    K218R
  • Entrez Gene
    RELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
  • Tag / Fusion Protein
    • T7 gp10 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer NTer primer 5'-GGGGGATCTTGGTGGCGTG-3'
  • 3′ sequencing primer CTer primer: 5'-CCCTGAAAACTTTGCCCCCTCC-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-RelA(K218R) was a gift from Warner Greene (Addgene plasmid # 23247 ; http://n2t.net/addgene:23247 ; RRID:Addgene_23247)

  • For your References section:

    Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-kappaB. Chen LF, Mu Y, Greene WC. EMBO J. 2002 Dec 2. 21(23):6539-48. 10.1093/emboj/cdf660 PubMed 12456660
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