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Addgene

hSyn-hCPT2-Halo
(Plasmid #232464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232464 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone size w/o insert (bp) 9270
  • Total vector size (bp) 11244
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CPT2
  • Alt name
    CPTASE
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1974
  • Entrez Gene
    CPT2 (a.k.a. CPT1, CPTASE, IIAE4)
  • Promoter hSyn
  • Tag / Fusion Protein
    • Halo (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer hSyn-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSyn-hCPT2-Halo was a gift from Timothy Ryan (Addgene plasmid # 232464 ; http://n2t.net/addgene:232464 ; RRID:Addgene_232464)
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