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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 23244 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
- Backbone size w/o insert (bp) 6655
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCerulean-2A-MYC
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Alt namecMyc
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SpeciesH. sapiens (human); synthetic fluorescent protein
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Insert Size (bp)2109
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequenced. These differences are not known to affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLM-mCerulean-cMyc was a gift from Michel Sadelain (Addgene plasmid # 23244 ; http://n2t.net/addgene:23244 ; RRID:Addgene_23244) -
For your References section:
Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, Tabar V, Mo Q, Studer L, Sadelain M. Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64. 10.1073/pnas.0904825106 PubMed 19549847