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pcDNA5 FRT to BioID-cDIC2-3xFlag
(Plasmid #232416)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232416 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5
  • Backbone size w/o insert (bp) 5738
  • Total vector size (bp) 7574
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dynein Intermediate Chain 2
  • Alt name
    DIC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1836
  • GenBank ID
    BC091498.1
  • Entrez Gene
    DYNC1I2 (a.k.a. DIC74, DNCI2, IC2, NEDMIBA)
  • Promoter CMV
  • Tags / Fusion Proteins
    • BioID (C terminal on insert)
    • Flag-3x (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5 FRT to BioID-cDIC2-3xFlag was a gift from Samara Reck-Peterson (Addgene plasmid # 232416 ; http://n2t.net/addgene:232416 ; RRID:Addgene_232416)

  • For your References section:

    The human cytoplasmic dynein interactome reveals novel activators of motility. Redwine WB, DeSantis ME, Hollyer I, Htet ZM, Tran PT, Swanson SK, Florens L, Washburn MP, Reck-Peterson SL. Elife. 2017 Jul 18;6:e28257. doi: 10.7554/eLife.28257. 10.7554/eLife.28257 PubMed 28718761
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