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Addgene

MKC83_HBB(HBBreg-HBA1)
(Plasmid #232409)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232409 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    KRD1
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hemoglobin Subunit Alpha 1
  • Alt name
    HBA1
  • Species
    H. sapiens (human)
  • Entrez Gene
    HBA1 (a.k.a. ECYT7, HBA-T3, HBH, METHBA)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.01.555926 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MKC83_HBB(HBBreg-HBA1) was a gift from Kyle Cromer (Addgene plasmid # 232409 ; http://n2t.net/addgene:232409 ; RRID:Addgene_232409)
  • For your References section:

    Dual alpha-globin and truncated EPO receptor knockin restores hemoglobin production in alpha-thalassemia-derived red blood cells. Chu SN, Soupene E, Wienert B, Yin H, Sharma D, McCreary T, Jia K, Homma S, Hampton JP, Gardner JM, Conklin BR, MacKenzie TC, Porteus MH, Cromer MK. bioRxiv [Preprint]. 2024 May 7:2023.09.01.555926. doi: 10.1101/2023.09.01.555926. 10.1101/2023.09.01.555926 PubMed 38766216