MKC83_HBB(HBBreg-HBA1)
(Plasmid
#232409)
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PurposeAAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232409 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneKRD1
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHemoglobin Subunit Alpha 1
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Alt nameHBA1
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SpeciesH. sapiens (human)
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Entrez GeneHBA1 (a.k.a. ECYT7, HBA-T3, HBH, METHBA)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.09.01.555926 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MKC83_HBB(HBBreg-HBA1) was a gift from Kyle Cromer (Addgene plasmid # 232409 ; http://n2t.net/addgene:232409 ; RRID:Addgene_232409) -
For your References section:
Dual alpha-globin and truncated EPO receptor knockin restores hemoglobin production in alpha-thalassemia-derived red blood cells. Chu SN, Soupene E, Wienert B, Yin H, Sharma D, McCreary T, Jia K, Homma S, Hampton JP, Gardner JM, Conklin BR, MacKenzie TC, Porteus MH, Cromer MK. bioRxiv [Preprint]. 2024 May 7:2023.09.01.555926. doi: 10.1101/2023.09.01.555926. 10.1101/2023.09.01.555926 PubMed 38766216