MKC89_CCR5(UbC-tEPOR-2A-YFP)
(Plasmid
#232404)
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PurposeAAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232404 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneKRD1
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTruncated Erythropoietin Receptor
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Alt nametEPOR
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SpeciesH. sapiens (human)
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Entrez GeneEPOR (a.k.a. EPO-R)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MKC89_CCR5(UbC-tEPOR-2A-YFP) was a gift from Kyle Cromer & Matthew Porteus (Addgene plasmid # 232404 ; http://n2t.net/addgene:232404 ; RRID:Addgene_232404) -
For your References section:
Enhancement of erythropoietic output by Cas9-mediated insertion of a natural variant in haematopoietic stem and progenitor cells. Luna SE, Camarena J, Hampton JP, Majeti KR, Charlesworth CT, Soupene E, Selvaraj S, Jia K, Sheehan VA, Cromer MK, Porteus MH. Nat Biomed Eng. 2024 Dec;8(12):1540-1552. doi: 10.1038/s41551-024-01222-6. Epub 2024 Jun 17. 10.1038/s41551-024-01222-6 PubMed 38886504