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KRD22_HBA1(HBA1reg-HBB-longHAs)
(Plasmid #232403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    KRD1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Hemoglobin Subunit Alpha 1
  • Alt name
    HBA1
  • Species
    H. sapiens (human)
  • Entrez Gene
    HBA1 (a.k.a. ECYT7, HBA-T3, HBH, METHBA)

Gene/Insert 2

  • Gene/Insert name
    Human Hemoglobin Subunit Beta
  • Alt name
    HBB
  • Species
    H. sapiens (human)
  • Entrez Gene
    HBB (a.k.a. CD113t-C, ECYT6, beta-globin)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KRD22_HBA1(HBA1reg-HBB-longHAs) was a gift from Kyle Cromer & Matthew Porteus (Addgene plasmid # 232403 ; http://n2t.net/addgene:232403 ; RRID:Addgene_232403)

  • For your References section:

    Gene replacement of alpha-globin with beta-globin restores hemoglobin balance in beta-thalassemia-derived hematopoietic stem and progenitor cells. Cromer MK, Camarena J, Martin RM, Lesch BJ, Vakulskas CA, Bode NM, Kurgan G, Collingwood MA, Rettig GR, Behlke MA, Lemgart VT, Zhang Y, Goyal A, Zhao F, Ponce E, Srifa W, Bak RO, Uchida N, Majeti R, Sheehan VA, Tisdale JF, Dever DP, Porteus MH. Nat Med. 2021 Apr;27(4):677-687. doi: 10.1038/s41591-021-01284-y. Epub 2021 Mar 18. 10.1038/s41591-021-01284-y PubMed 33737751
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