Skip to main content
Addgene

pHR-TetOn-mCherry-OAZ1_FS-YFP
(Plasmid #232356)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232356 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    mCherry after doxycycline induction

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OAZ1 derived polyamine sensing module
  • Alt name
    OAZ1_FS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    170
  • GenBank ID
    NM_004152.3
  • Entrez Gene
    OAZ1 (a.k.a. AZ1, AZI, OAZ)
  • Promoter TRE3G
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • eYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GGACGAGCTGTACAAGGGCTC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.08.24.609500 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-TetOn-mCherry-OAZ1_FS-YFP was a gift from Ankur Jain (Addgene plasmid # 232356 ; http://n2t.net/addgene:232356 ; RRID:Addgene_232356)

  • For your References section:

    Genetically encoded fluorescent reporter for polyamines. Sharma P, Kim CY, Keys HR, Imada S, Joseph AB, Ferro L, Kunchok T, Anderson R, Yilmaz O, Weng JK, Jain A. bioRxiv [Preprint]. 2024 Nov 17:2024.08.24.609500. doi: 10.1101/2024.08.24.609500. 10.1101/2024.08.24.609500 PubMed 39253442
Related items: