mCherry-DRP1 K38A
(Plasmid
#232350)
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PurposeExpresses K38A variant of human DRP1 with mCherry tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemCh-alpha tubulin (Addgene Plasmid #49149)
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Backbone manufacturerGia Voeltz Lab
- Total vector size (bp) 6783
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDRP1
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SpeciesH. sapiens (human)
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MutationK38A
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Entrez GeneDNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (N terminal on backbone)
Cloning Information
- Cloning method Other
- 5′ sequencing primer mCherry-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byJonathon Friedman, Gia Voeltz Lab: mCh-Drp1 (Addgene Plasmid #49152)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-DRP1 K38A was a gift from Samantha Lewis (Addgene plasmid # 232350 ; http://n2t.net/addgene:232350 ; RRID:Addgene_232350) -
For your References section:
A spatial atlas of mitochondrial gene expression reveals dynamic translation hubs and remodeling in stress. Begeman A, Smolka JA, Shami A, Waingankar TP, Lewis SC. bioRxiv [Preprint]. 2024 Aug 17:2024.08.05.604215. doi: 10.1101/2024.08.05.604215. 10.1101/2024.08.05.604215 PubMed 39149346