GFP-hRhoD
(Plasmid #23235)

• Depositing Lab: Channing Der
• Publication: Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-63.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RhoD
• Species: H. sapiens (human)
• Insert Size (bp): 630
• Entrez Gene: RHOD (a.k.a. ARHD, RHOHP1, RHOM, Rho)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry